FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p23c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p23c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763799
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18300.018.902711
TATACAG27550.017.7755185
GGACAGC7451.8189894E-1216.4328126
TAGAGCG5753.8216967E-916.280047
ACCTAGG4451.1599586E-616.1947361
GAGCGCA6006.724804E-915.6000099
GTATATA52750.014.8914821
TATACGG14050.014.86471755
TCCTAGG5354.848389E-714.8128642
GTATAGA20450.014.800961
CTAGGCG4502.3280141E-514.4000089
GCCGACC10350.014.323392140-144
TTATACT29250.014.2823614
CCTAGGA6101.3783028E-714.17266752
TATAGGG11850.013.9832982
CCTGGAC6702.8598151E-813.9774963
CTAGGAT9800.013.9665243
TCGTGCG3100.00612254913.9354939
GGGAGCG3650.001129523413.8097297
TAGTCCG9457.2759576E-1213.7142949
Produced by FastQC (version 0.11.2)