FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p22e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p22e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4002460
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG9950.020.266875
GTATAGA29500.016.8568951
GTATAGG22500.015.69511
GTATTAG25900.015.5825591
GCCGACC14250.015.35101140-144
TACACAG22100.015.31642155
CCGCGTA4351.6905899E-514.89654359
TACACCG12100.014.8801175
GTATAAT49350.014.7497531
CTAATAC32250.014.5181543
ACCGTGC7606.87578E-1014.2114078
TAGGTAG13850.014.0399555
CCGTGCG3600.001018725313.9999939
TAATACT57950.013.92013554
CTGTGCG6251.8688479E-713.8239939
GTATATA72100.013.5942161
TAGACAG17050.013.5169085
CTTAGGG8552.764864E-1013.4797443
CTAGGGT8552.764864E-1013.4782274
TTATACT47050.013.47106554
Produced by FastQC (version 0.11.2)