FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p21b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p21b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324361
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG23100.020.5773965
TATACCG17800.019.8259745
TATACAG38600.018.2850955
GTATTAG28600.017.3853281
GCCCGGC14950.016.8552259
GTATATA77850.016.8466111
GTACTAG19350.016.3859441
CAGCGTG8900.015.3699089
GAGCGCG4301.5163778E-515.0689069
GCCGACC16650.014.952795140-144
TATAATA100900.014.9222472
GTATAGG24250.014.8579221
CTACCCC11750.014.7114994
GGGCCCG17700.014.6452657
GTATTAA45450.014.5865881
TATATAC84450.014.5859713
GGCCCGG17800.014.5623148
TCCGGGG19200.014.2567733
ATACAGG29300.014.2557046
CCCTTGC16250.014.1789648
Produced by FastQC (version 0.11.2)