FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p212g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p212g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459353
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGG5351.4697434E-917.494419
TATACCG14450.017.4438275
TAGACAG13300.016.7861735
CTAATAC27050.016.2432653
TATACAG33150.016.0764395
GTATAGA25200.016.0139831
GTATATA66300.015.4342921
CTACCCC8900.015.3759834
GTCCTAA13300.015.1711421
CTAGGAC9050.015.1222273
GGACAGC8701.8189894E-1214.8990016
ACTCCGC7806.366463E-1114.7697358
TATATAC69800.014.6535773
CTATACT27250.014.5370244
GCTATAG13900.014.5162731
GTGTAGG9950.014.4850091
TAATACT54800.014.3260114
CTGTCCG5557.398794E-714.2695219
GTATAGG18750.014.2204171
GCCGACC11650.013.958936140-144
Produced by FastQC (version 0.11.2)