FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p210c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p210c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125713
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG21700.021.9043375
ACTCCGC4803.45608E-1019.4991238
GCGTCAG4955.14774E-1018.9233841
GTATTAG13350.018.890671
ATACAGG16050.018.843826
TACACCG6151.8189894E-1218.7365995
GTATATA43150.018.7024211
GACCGTC5459.458745E-1118.4945817
TATACCG10800.017.337865
ATACAGT15950.016.704596
CAGGGGT3103.263924E-416.2646064
TAGCCCG3153.6908983E-416.0041775
ATTATAC28150.015.8657793
GTATAGA16450.015.7687481
TACACAG13250.015.7626065
AGTACCG11000.015.7131925
GCCGACC6850.015.548772140-144
GTATAGG12700.015.3186551
ACTGACC5655.434231E-815.2913498
CCTAGAG5203.514033E-715.2394092
Produced by FastQC (version 0.11.2)