FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p18e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p18e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015086
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG3601.2676173E-719.9997779
TAGACAG17000.018.21615
TATACAG34450.017.9781555
GTATAGG18250.017.7691671
GTATTAG25900.016.4160881
TATAGGG17150.016.3821812
CTGTCCG7151.6370905E-1116.111719
GTATATA71050.016.025461
CTATACT30000.015.6042974
GGACCGT7853.6379788E-1215.595686
TATAATA83350.015.4710092
GTATAGA28200.015.3327331
GTCCTAA14200.015.2247561
TCTATAC35450.015.0363853
TATACTG53650.014.9000995
TGGACCG10400.014.5419235
GTATTAA34700.014.1220221
TTATACT47550.014.0858984
CCTATAC17900.014.0845483
TACACCG12300.014.0521265
Produced by FastQC (version 0.11.2)