FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p17h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p17h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776884
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGC3101.5581982E-518.5814198
GTATTAG28400.017.252271
GGACCGC3855.3037547E-616.8329856
TATACAG34150.016.0257915
TATACCG18600.015.4861585
GACCGCG5253.94175E-715.0865427
CCTATAC19950.014.8011383
GTATTAA43150.014.5276081
ACCGCGC4001.3812848E-414.40068
GTATATA70100.014.2873751
GTATAGA28900.014.2112631
TCTATAC28800.014.0039233
TAGTCCG13950.013.9356969
GTATAAT49550.013.8145151
CTGATCG8903.6379788E-1113.7530189
TAGGGTG9609.094947E-1213.5019945
CTATACC25350.013.3519694
CTTAGGG8653.3469405E-1013.3216513
CCCTAGG4905.152946E-513.2343351
GCCGATC6553.305977E-713.191398
Produced by FastQC (version 0.11.2)