FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p17c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p17c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488574
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA3754.1305993E-617.280689
TAGGTAG14700.017.1484495
GTATTAG26550.017.1020971
TATACGG20900.016.885895
CCGTGCG4058.587618E-616.000639
TCTATAC31150.015.957733
TATACAG42600.015.7234385
GTATATA79400.015.43127251
TATACTG43050.014.8898745
GTATAGA28300.014.5164941
GAGCGCA9255.456968E-1214.0113629
CATATAC45250.014.0101963
GGACTGC9405.456968E-1213.7909416
GTATAAT46650.013.5957871
ATACAGG30750.013.584146
ATACGGT22850.013.2376956
CTATACT32100.013.0139644
ACTAGAC20100.012.9028773
TATATAC89350.012.900473
CTGTGTG10653.6379788E-1212.8455769
Produced by FastQC (version 0.11.2)