FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p15g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p15g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931497
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC9100.019.7881163
ACCTCGC5051.3627869E-817.1098988
GCGTCAG8250.016.5960921
TAGACAG14550.016.3344635
GTATTAG20500.016.1700041
TATACGG16200.016.0044755
GTATTAC26700.015.9238061
CCGCGCA3707.110513E-515.5674039
ACGGTGT14600.015.2885538
CCTACAG12900.015.0757833
CTAGGAC6701.7680577E-915.0507823
GTATATG32800.014.9396761
TAGGACC8701.8189894E-1214.9012284
CTAGACA12600.014.8618064
TCTATAC22400.014.7916173
GTATAGG14000.014.4123951
GTATATA55550.014.3994231
CTTAGGG6552.1273081E-814.2957823
TATACAG30150.014.09349255
CCTCGCG5155.346814E-613.9804349
Produced by FastQC (version 0.11.2)