FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p14h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p14h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922264
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA67050.019.017981
GTATTAG21500.017.0891951
CTAGGGT7151.6370905E-1116.1161944
TATACCG16400.015.8088325
CCTCGCG3707.110274E-515.5674639
TAGGGTG7453.092282E-1115.4669545
TACACCG10250.015.4575245
CTAATAC24350.015.0846283
GTATAGA23300.014.8414121
TCTATAC25100.014.6338923
TATAATA78150.014.4700922
GTGTTAG17500.014.4085371
TATACAG31500.014.4036025
GCGTCAG8101.1641532E-1014.2306531
GTATAAT40050.014.0307851
CTCGGAG7708.3491614E-1014.0294825
CCTACAC9851.8189894E-1213.8925123
CATATAC34400.013.8181173
CCATGCG10750.013.3952589
CCCTAAG7601.0397343E-813.271021
Produced by FastQC (version 0.11.2)