FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p13g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p13g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720952
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA49450.018.0701271
TATACGG14900.017.4006845
TATACAG28400.016.7369255
TCTACAC17450.016.5115013
CTGATCG6608.185452E-1116.362059
TATACCG13500.016.0043335
GGACCGT5905.3696567E-915.8675356
CTACACT12350.015.7457334
ACGGTGT13000.015.5067588
GTGTAAG10700.015.4899461
TAGGACC8850.015.4623814
AGACTCG6601.4333636E-915.2768645
GGACTGG9450.015.24116
GAGTCCG6301.2823875E-814.8557019
GTATAAA37250.014.7025661
TATATAG37500.014.5959525
ACCGCTC5003.9385395E-614.3991328
GTAGGAC6552.1260348E-814.2963133
TACGGTG13700.014.1904927
TCTAGAC10700.014.137013
Produced by FastQC (version 0.11.2)