FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p13d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p13d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10152521
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG70300.017.6290361
GTATATA175200.017.0263561
TATACAG89100.015.4376035
GTATAGA69800.015.2778331
TCTATAC84950.014.668373
GTATAGG44650.014.2009271
CTATACT75950.014.0341444
ACCTCGC18100.013.9227798
GTATTAA102300.013.73454951
TATACTG125850.013.7335325
TAGACAG49150.013.4799585
CTAGACT32600.013.2551814
TAGGTAG38250.013.1792535
GCCGACC27250.013.151752140-144
ACCGTGC18650.013.1261248
GTCTTAG39800.013.0348151
TAATACT138450.012.9526434
TATAATA220500.012.8713222
TATATAC186250.012.6072533
TATACCG45700.012.6066015
Produced by FastQC (version 0.11.2)