FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p12f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p12f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344006
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG30150.021.7358285
TATACCG14750.018.0647475
GTATAGA24400.018.0126081
GTATATA59250.017.1461811
GTATTAG22250.016.8387531
GTATAGG19500.016.6270241
ATACAGG24950.015.0086596
AGTACCG18550.014.7523715
TAGGCAG8901.8189894E-1214.5648035
TATATAC64350.014.4389223
TAGGGTG7505.620677E-1014.402975
TATACGG15450.013.9834675
TCTATAC25850.013.9316663
TAATACT48200.013.8953994
CTATACT25850.013.6511944
ATATACA59200.013.6248414
TAGTACT22450.013.4731174
GTACCGC11250.013.4423716
GACTGCG8101.7862476E-913.3346887
TATAATA75050.013.244682
Produced by FastQC (version 0.11.2)