FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p11d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p11d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126067
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCG4151.193257E-920.818639
ACCGCGC3751.9480831E-719.2000168
GCTCGGA5552.3774192E-916.8729823
TAACCCG3554.9817674E-516.2290025
GGCTCGG4901.7875209E-716.174672
TATACGG16950.016.1452245
GTATTAG20150.016.0951331
TATACAG24450.015.9054185
TATACCG14200.015.7218465
GTATATA45200.015.4664411
ACAGCGC6751.9754225E-914.9333478
GTCTATA17500.014.8259161
GTCCTAG5909.128962E-814.6583921
ATACCGT20300.014.5442126
CCCGTGC3000.00492618514.4000138
TATAATA59650.014.2531362
CTATACT21850.014.1738864
ATACTAT36500.014.0077336
CACAGCG7255.4078555E-913.9036847
GGTCCGC4753.874309E-513.6421178
Produced by FastQC (version 0.11.2)