FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p112c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p112c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235958
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG12100.023.2130365
GTATTAG24800.019.179351
GGCTCGG4506.785922E-817.6089573
TATACAG30800.017.3034925
AGACCGC7551.8189894E-1216.2154066
GTATAGA25400.016.172711
GTATATA64250.015.9278391
TAGACCG12350.015.7452495
GTATAGG17800.015.3852751
GTATAAG26200.014.8536821
CTCGGAG9750.014.7733215
TATATAC71650.014.6787863
CTATACT27600.014.6138714
CGGAGAT14350.014.5502767
GTATATG33850.014.4774441
GCTCGGA7003.2850949E-914.4051014
ATACAGG21000.014.4030966
TAGGGTG9203.6379788E-1214.0908575
GTCCCGA3600.001011419514.0131941
TATAAGG25200.014.008862
Produced by FastQC (version 0.11.2)