FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p111d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p111d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6386017
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG13800.016.7095031
GTATAGA46700.016.6647781
GTATATA117400.016.3269941
GTATTAG44350.015.598051
GCTCGGA12400.014.5205444
TACACCG19100.014.3279545
TAGACAG27750.014.0140855
TATACAG57550.013.8902995
TATATAG76600.013.6324275
GCCGACC16150.012.922618140-144
GTATACG37600.012.8404311
GCGTCAG15300.012.7164251
TATATAC128700.012.6492843
TATACGG34650.012.4704465
CCTAGAC17400.012.4196133
ATTATAC87000.012.4196133
CTGATCG14550.012.3705359
GTCTAAG25150.012.3203531
TATAGTG47050.012.2451565
GGACTGC17700.012.205386
Produced by FastQC (version 0.11.2)