FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p110b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p110b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7572942
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA163800.019.3109281
TATACAG84550.017.4617985
TATACCG38450.017.2321785
GTATTAG53550.017.222771
TACACCG23050.016.5597445
TCTATAC66550.014.9363
TATAATA196400.014.9281162
CTATACT66450.014.848614
CTCGGAG22350.014.50052455
TAGAGCG20150.014.2940797
TAGGTAG28850.014.2291115
TATATAC182200.014.1526553
ATTATAC101500.013.8379523
GCTCGGA14750.013.6718164
TATTATA208450.013.5813432
TAGGACA27500.013.3566544
ATATAAT186250.013.3467451
TAGACAG29800.013.2921475
GTATTAT127500.013.2238581
ATACAGG62000.013.1253136
Produced by FastQC (version 0.11.2)