FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mah58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mah58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408567
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG15850.019.0844865
GTATTAG22450.017.9760271
CCGTGCC5051.3631507E-817.1096829
CTATACT27000.016.005894
GTATATA68600.015.9676671
CTCGGAG10450.015.1623615
TAGGTAG11150.014.8563945
GCGTCAG10000.014.4129211
TATATAC71650.014.376413
GTATAAT41250.014.1508681
GTAGGAC7759.1677066E-1013.9418433
TCTATAC26000.013.8524713
CTAATAC26650.013.7848993
CTAGGAC8903.6379788E-1113.7590843
TAGACAG14750.013.6718255
TAGACCG9507.2759576E-1213.6461255
GTATAGA25950.013.6075751
TATAGTG22900.013.5236365
TCAGGAC15100.013.3570863
TATAATA85200.013.274832
Produced by FastQC (version 0.11.2)