FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mah4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mah4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3453253
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG16500.017.8960955
CTTACAC14500.017.388383
CAGGGGT6909.094947E-1216.7012184
TAGTCCG8200.016.6836539
GGCTCGG4751.2574128E-716.6823863
GTATTAG24350.016.573411
TATACGG20750.015.9660735
CCCGTGC4551.4760681E-615.8250938
GTATATA57050.015.6635011
TATACAG29750.015.4935655
GCTCGGA6751.9663275E-914.9383124
CTGTGCG5858.320967E-814.7698739
TCGGAGA14650.014.7474466
ATACGGT21050.014.3691276
CTCGGAG10250.014.0528535
GCCGACC10850.013.927049140-144
TAATACT46050.013.919954
TATAATA71000.013.9016182
TCAGGGG7305.9153535E-913.8154263
ACTGTGC13750.013.6153358
Produced by FastQC (version 0.11.2)