FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mah33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mah33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125315
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21400.019.1899071
CTAATAC22600.017.8458983
GGCTCGG5401.6480044E-917.3444841
TAGACAG14150.017.3023385
TATACCG13450.017.1320765
ATACCGT19700.016.4483246
GTATATA55800.016.0102941
TATACAG29600.015.5693535
TAGGGGT7002.0008883E-1015.4305894
CTATACT22500.015.3620084
ACCGTGC5253.944151E-715.0855138
GTATAGA22950.015.0685121
GTCTAGG8301.0913936E-1114.7564761
ACTATGC19650.014.6562928
TAATACT40350.014.6338834
CTCGGAG9400.014.554865
TATAGGG12950.014.4604992
TAGGTAG10400.013.8477435
CTATACA22400.013.8232364
TATAATA69100.013.6544092
Produced by FastQC (version 0.11.2)