FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mah148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mah148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3237213
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG9600.021.0163781
GTATTAG22850.019.5513441
GTATAGA24250.017.234051
TATACGG18350.016.0902845
CTGTGCG5503.9051884E-815.7089549
GCCGACC10150.015.314506140-144
TAGGGAG7654.7293724E-1115.0617185
GTATATA59150.014.9837841
GTCTTAG13050.014.9081711
CTTACAC15350.014.5457593
GTATAAT40400.014.2685461
TATAATA78550.014.2139772
ACTGTGC13350.014.0227828
TATACAG29400.013.7169225
TAGGTAG12600.013.7169215
TTATACT36650.013.5584864
TCCTATA20700.013.2234062
GCAGTGC9307.4578566E-1113.1615818
GTATAGG15350.013.1437961
GTCCTAG6051.9360596E-613.101121
Produced by FastQC (version 0.11.2)