FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mah137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mah137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544403
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18250.020.139731
CCTAGGG2557.311837E-519.7757643
TATACTG30900.016.5509935
GTATATA47000.016.4071161
GTATAGA19150.015.8061781
TCTATAC20700.015.3129113
TATACCG11500.015.0327215
GCCGACC8750.014.802009140-144
ACAGTCC8451.4551915E-1114.4870418
ACTAGGG6001.12682756E-714.4080573
CTAGTAC10000.014.4080573
TGTACCG7154.4201443E-914.1041255
CTAATAC21550.014.0403353
TCCTAGG5256.480206E-613.7227692
ATACCGT15500.013.4761196
CTATACT19500.013.2984384
CTAGGGA8703.6379788E-1013.2474854
GGACCGT4955.6857458E-513.0959156
TACTGTG16600.013.0136977
GGCTAAG7207.302333E-813.0123951
Produced by FastQC (version 0.11.2)