FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_matalpha2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_matalpha2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7529185
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG53250.020.0184441
GCGTCAG23900.017.1741684
GTATAGA63550.016.5472371
GTATATA158250.016.1119371
TAGGTAG33400.015.3077245
TACACCG23950.014.7329385
ACTATGC48550.014.383838
TAATACT118600.014.1471834
TCTATAC61150.013.89582353
TATACAG75450.013.8390895
TAGGACA30250.013.8070654
CTAGGAC19400.013.7340543
GTATAAT101100.013.67849351
CTAATAC64400.013.5300143
CTATACT64950.013.304574
TTATACT97400.013.2340524
TATACGG43550.013.2281945
TATACTG94850.013.2102385
TAGACAG35650.013.1296335
TAGACCG24850.013.0402185
Produced by FastQC (version 0.11.2)