FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_matalpha1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_matalpha1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4290050
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG13500.028.8046764
CAGCGTC18700.020.4106832
GCAGCGT17100.018.9535371
AGCGTCA21950.018.043933
TATACCG18250.017.7563065
TATACAG42750.016.6763925
CTAATAC35300.016.523933
ACCGTGT12200.016.5230358
CGTCAGA25500.016.379135
GTATATA79400.014.8763781
GTATAAT54600.014.3783061
GTATAGA29500.014.1605421
AGATGTG33450.013.9897159
GTATATG43850.013.7969651
ATACAGT36800.013.3065086
TATAATA118050.013.2988392
TAGGTAG17400.013.2435295
GTATTAG31650.013.1986091
CAGATGT33300.013.1879488
TATATAC87050.013.1531983
Produced by FastQC (version 0.11.2)