FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mata2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mata2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4671619
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG23400.049.5428244
GCAGCGT22600.044.9339331
CAGCGTC27100.041.9815562
AGCGTCA31600.036.6863823
CGTCAGA35900.032.292545
GTCAGAT43050.027.4309866
AGATGTG45200.026.7588379
CAGATGT48450.024.666688
TCAGATG49400.023.9008467
GTATTAG35600.020.4331421
GTATAGA36500.019.337351
TAGGTAG17550.019.283755
TATACCG21050.017.1036435
TATACGG28000.016.9729235
CTAATAC38100.016.6312083
GTATATA93800.016.5849691
TAATACT66200.015.7717814
TCTATAC34500.015.4446733
CTATACT34800.015.3116934
TATACAG47900.015.1829665
Produced by FastQC (version 0.11.2)