FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mata1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mata1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470780
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG17450.042.9108054
CAGCGTC17900.041.834452
GCAGCGT15550.039.828771
AGCGTCA21850.034.270223
CGTCAGA25350.029.822235
GTCAGAT30850.025.2056166
CAGATGT31900.024.5984788
AGATGTG33000.023.7785329
TCAGATG34000.023.7143697
GTATAGA27350.016.8520031
GTATAAT43650.015.1786261
ACCGTGC5706.06542E-815.1557998
TATACAG33900.015.0795155
TATACGG17700.015.0507165
GGACCGT6701.7789716E-915.0446466
CCTAGAC9650.014.9223663
TAATACT51300.014.5963654
TAGACAG15500.014.3998775
TAGGTAG15050.014.35203555
GTATATA64750.014.3475871
Produced by FastQC (version 0.11.2)