FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p28c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p28c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4622844
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG17900.039.824494
CAGCGTC21750.032.114712
GCAGCGT20000.032.0519831
AGCGTCA22900.031.1291853
CGTCAGA30200.023.6045825
GTCAGAT35600.020.428646
AGATGTG37000.020.2362129
CAGATGT37850.019.4013468
TAGGTAG19950.017.6856585
ACCGTGT10900.017.172938
TCAGATG42800.016.8210777
TATACCG21200.015.9635725
TATACAG45300.015.8953415
TACACCG13400.015.58336455
GTATTAG30750.015.2252061
GTATATA88100.015.0430831
GTATAGA35800.014.8882491
CTAGGAC9250.014.7904013
GTATATG52900.014.4326181
GTCTAGG12100.014.2863331
Produced by FastQC (version 0.11.2)