FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p27g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p27g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4582050
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG18650.037.4518974
GCAGCGT18850.035.151791
CAGCGTC20350.033.6188432
AGCGTCA25200.027.7173773
CGTCAGA28350.024.8916655
CAGATGT36800.020.5417358
GTCAGAT36700.019.424516
AGATGTG39750.019.0172529
TAGGACA16900.017.8954874
TCAGATG42100.017.1006857
TAGTCCG20100.016.834399
GTCTAGG11700.016.6206861
GTATAGA32050.016.4046081
ACTGCGC10550.016.3777568
TATACAG40700.016.2769995
GTATTAG30300.016.1635681
GTATATA81550.016.0737931
GACCGTC14800.016.0526897
ACCGTGT10050.015.7598558
TACACCG12400.015.6791675
Produced by FastQC (version 0.11.2)