FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p25e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p25e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2910599
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11000.045.1667184
GCAGCGT12250.034.6900521
CAGCGTC15700.030.2727032
AGCGTCA17450.028.4723453
CGTCAGA20500.024.9382865
ACCGCGC3004.880094E-721.5977638
GTCAGAT26000.021.047596
GTATAGA21750.020.862691
AGATGTG25400.020.6907719
TATACAG28600.020.6447665
CAGATGT26100.020.4116788
GTATTAG21450.019.4755481
ATACAGG23500.018.9970426
TCAGATG28000.018.7694857
ACCGTGC5301.2987584E-917.6585488
GTATATG29050.017.3556521
TACAGGC13800.017.2156097
GTATATA63650.016.973911
GTAGGAC6155.275069E-1016.3916453
GTCCTAG5854.78758E-916.0057681
Produced by FastQC (version 0.11.2)