FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p23c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p23c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763799
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11600.039.7342874
GCAGCGT12400.032.532681
CAGCGTC13650.032.185792
AGCGTCA16400.028.104743
CGTCAGA19950.023.4646425
CAGATGT23750.021.219188
AGATGTG24750.019.7800719
GTCAGAT24450.019.7351026
GTATAGA19250.018.7108171
TCAGATG27450.018.0967627
GTATATA53050.017.2453421
TATACAG28050.016.6887555
CCTTGCG12300.016.3887969
TATACCG13200.015.8222245
GTATTAG18150.015.4789491
CTATACT20750.015.2713714
CTAATAC20500.015.10629753
GACCCGT3406.595197E-414.8273166
TCTATAC21000.014.7466243
GTATAAT31450.014.65928651
Produced by FastQC (version 0.11.2)