FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p22e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p22e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4002460
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG16350.041.3970454
GCAGCGT17700.034.5853161
CAGCGTC20700.032.002772
AGCGTCA25600.026.4387973
CGTCAGA27950.024.9890175
GTCAGAT34400.020.5128926
AGATGTG36050.020.1704549
CAGATGT35050.020.1297158
TCAGATG38400.018.3736067
TAGACAG17200.017.1638495
GTATATA67500.015.3640181
GTATTAG27250.015.3287791
TTATACT47250.014.6294734
TAATACT56000.014.1437284
TATATAC79150.014.1005033
TATACGG21450.013.7630855
ATACAGG27750.013.7521996
TATTATA102750.013.6654172
GTATAGG21150.013.6205841
TATACAG38400.013.5008325
Produced by FastQC (version 0.11.2)