FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p21b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p21b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324361
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG19500.043.5800744
GCAGCGT18950.040.6710551
CAGCGTC23350.036.3957562
AGCGTCA25250.033.656293
CGTCAGA31050.028.2968945
AGATGTG35850.024.700799
CAGATGT36150.024.4958068
GTCAGAT36700.024.1367956
TCAGATG38700.023.067777
TATACAG37800.020.0049765
TACACCG12250.017.6370415
GTATTAG30000.017.0471
GTATATA76700.016.9978541
TATACCG17900.016.4957245
AGTCCCG6557.2759576E-1116.4871238
CTACGCG2700.00244478215.9986159
GTATAGA31850.015.152221
GGACCGT6251.1497832E-814.9797266
GTATAAT48800.014.9077621
CGTAGAC10350.014.6124993
Produced by FastQC (version 0.11.2)