FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p212g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p212g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459353
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG10350.029.2208614
CAGCGTC14850.019.8811472
GCAGCGT14900.019.3339481
TACACCG9900.018.1839775
CGTCAGA19050.017.3878925
TATACAG29750.016.7011415
GTATTAG20900.016.540241
AGACCGC7001.0913936E-1116.4590976
GTATAGG19700.016.0854531
AGCGTCA18450.016.00193
TAGACAG13200.015.2745415
TTATACT39200.014.6956234
CTAGGGT7857.094059E-1114.6769024
CAGATGT24900.014.4568328
CTAGGAC7003.2960088E-914.40171053
GTATAAT40050.014.3858111
AGATGTG25050.014.3702639
GTCAGAT23900.014.1606776
TAGTCCG13750.014.1372049
CCCTAGG4602.8573988E-514.0906661
Produced by FastQC (version 0.11.2)