FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p210c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p210c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125713
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG7850.031.189344
GCAGCGT8250.027.0611041
CAGCGTC9000.026.4045372
AGCGTCA11750.020.8371333
GTATATA42750.018.5308071
ATACAGG14850.018.4269756
TATACAG22350.018.3651395
CTACCCC5401.6589183E-917.3359054
AGATGTG16150.016.9397059
CGTCAGA14950.016.8586885
GTCCTAC3855.294776E-616.835251
CAGATGT15650.016.5608628
GTATAAT25100.016.3545631
GTATAGA16150.016.0534271
CCTGTCC5854.813046E-915.998618
TATACGG11450.015.7228565
TTATACT25000.015.5543064
TCTATAC16850.015.3850723
CTTACAC8501.8189894E-1215.249323
TACAGGC10400.015.2294467
Produced by FastQC (version 0.11.2)