FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p18e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p18e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015086
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG20300.048.6019824
GCAGCGT16650.048.4510961
CAGCGTC19550.048.25692
AGCGTCA23600.041.500793
CGTCAGA29250.033.9768145
GTCAGAT32900.030.4262476
AGATGTG37150.027.9060179
CAGATGT36650.027.697428
TCAGATG39050.025.8107837
TATACAG35100.018.0553115
ACCTCGC7800.017.5369158
TATACGG22900.017.2964365
GTATTAG25000.016.9985471
GTAGGAC9750.016.9884073
TACGGTG20950.016.8386127
GTATATA71250.016.78121
TACACCG11900.016.3397835
ACCGCGC4058.5986685E-615.99858958
ACGGTGT20500.015.8034848
ATACGGT24100.015.5387386
Produced by FastQC (version 0.11.2)