FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p17h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p17h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776884
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG12300.019.31884
TATACAG38450.018.7272915
GTATTAG25300.018.5033511
TATACCG18100.017.5043265
TACACCG11450.016.9796855
GTATAGA29650.015.5457911
ACTATGC23450.014.7365218
GTATATA72000.014.7042331
CAGCGTC16850.014.5298742
GTCCTAG9500.014.4041481
TCTATAC29000.014.4012883
GCAGCGT14150.014.2514531
ATACAGG27900.014.1948186
TATAATA114150.013.8780862
TATATAG50100.013.7976415
GTGTAAG15700.013.7619251
TAGGTAG15200.013.73807055
ATAATAC62050.013.6934073
TAATACT57900.013.6799794
CTAGGAC7901.2296368E-913.6721093
Produced by FastQC (version 0.11.2)