FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p17c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p17c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488574
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG13350.040.452954
CAGCGTC18700.029.2649922
GCAGCGT16750.028.8066311
AGCGTCA23200.023.5879173
CGTCAGA25800.022.0484265
CAGATGT30350.019.4513918
AGATGTG29800.019.3272119
GTATATA79850.018.4889131
GTCAGAT32950.018.1381456
GAGCGCA10050.016.4762089
TCAGATG35500.016.426777
GTATTAG24400.015.9380941
GTATAAT43450.015.9116021
TATACAG42600.015.7196735
TATACCG18300.014.9521185
ATACAGG29600.014.8391276
CCGCACG4452.0970314E-514.5605579
TCTATAC30000.014.4010443
CATATAC43750.014.3187513
CTAATAC30950.014.1916583
Produced by FastQC (version 0.11.2)