FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p15g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p15g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931497
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG14150.039.6987724
GCAGCGT13450.037.4883421
CAGCGTC15550.035.6626932
AGCGTCA18800.030.2627373
GTCAGAT23300.025.6543566
CGTCAGA22950.024.790395
CAGATGT27600.021.6500938
AGATGTG28800.020.9979829
TCAGATG29550.019.9777777
GTATAGA23100.019.021221
ACAGCGC7550.017.1639168
TACACCG8150.016.789395
TATACAG27200.015.8862485
TAGGACG5956.000846E-915.7349494
TATAGGG11800.015.2584972
ACCGCTC5253.9476072E-715.0842658
TATACGG16000.014.8536415
GTATACG20950.014.784441
CCTATAC12950.014.4591423
GTATTAG18950.014.4442451
Produced by FastQC (version 0.11.2)