FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p14h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p14h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922264
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG14000.036.517054
CAGCGTC14400.035.5032922
GCAGCGT14100.034.2212141
AGCGTCA18650.027.4117933
CGTCAGA21500.024.783275
AGATGTG26150.020.6491859
GTCAGAT27300.020.0454736
CAGATGT26800.019.879728
GTATTAG20700.018.7872451
GAGCGCA7550.018.1184089
TCAGATG31100.017.362587
ACCGCGC2500.001460852217.2792388
TATACCG15250.016.5258435
TAGGACT10700.016.1507554
GTATATA62050.015.66865351
TACACCG8750.015.6354695
TATACAG32150.015.4537365
CCTATAC17150.015.1145883
GTATATG35700.014.9280551
ATACAGG23200.014.8976796
Produced by FastQC (version 0.11.2)