FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p13g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p13g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720952
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG13100.037.9327554
CAGCGTC13750.034.5689242
GCAGCGT12100.034.5263821
AGCGTCA16500.029.679843
CGTCAGA21600.023.672345
GTCAGAT24300.021.6348156
AGATGTG26300.020.8041889
CAGATGT25650.020.2086858
TAGACAG10650.019.6103345
TCAGATG26750.019.3776747
GTATTAG19950.016.6082321
TTAGACT16100.016.103244
TAGGTAG10300.016.0815265
TATACAG28150.015.8617065
TAGACTC11150.015.5014735
GTATAGA20950.015.4716611
CTTACAC11300.014.65838053
CTTAGAC12900.014.5151073
GTATATA54350.014.4455931
GTCCTGG12050.014.346061
Produced by FastQC (version 0.11.2)