FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p13d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p13d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10152521
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG45550.045.3735734
GCAGCGT46050.038.3210951
CAGCGTC53450.037.8600232
AGCGTCA63400.032.8258443
CGTCAGA72850.028.9632665
GTCAGAT88750.024.26126
AGATGTG92750.024.0626729
CAGATGT97000.022.3403938
TCAGATG100400.021.512147
GTATTAG68800.017.1694681
CCGCGCG10000.015.8386719
GTATAGA77900.015.3487171
TATACAG83350.014.8604615
GTATATA170400.014.7099471
TACACCG30400.014.6868125
TATACGG54100.014.509045
ACCGTGC18150.014.2797958
GTATAGG46100.014.0618691
GTCCTAG22700.013.64407351
TAGACAG46050.013.6050175
Produced by FastQC (version 0.11.2)