FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p12f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p12f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344006
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG13800.039.135534
GCAGCGT14900.030.4528371
CAGCGTC16750.030.0957182
AGCGTCA18600.029.0364693
CGTCAGA23700.023.0916125
AGATGTG27000.021.064379
GTATAGA21450.019.8106251
CAGATGT29000.019.3634038
TATACAG31000.018.815355
ATACAGG22750.018.6749576
TCAGATG30750.018.2614217
GTCAGAT31250.017.973546
GAGCGCA6950.017.6095899
CTAGGAC7850.016.5119483
TAGACAG15300.016.4727335
GTATTAG23600.016.174741
GACTAGG5905.3933036E-915.8626768
TATTATA76350.015.4687892
TAGAGCG8850.015.4559417
GTATTAT49400.015.4544091
Produced by FastQC (version 0.11.2)