FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p11d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p11d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126067
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG14300.044.8182534
GCAGCGT13700.043.1058241
CAGCGTC15400.041.6176152
AGCGTCA19850.032.2872053
CGTCAGA22350.029.641785
GTCAGAT25700.026.3383626
CAGATGT27600.025.0418178
AGATGTG28350.024.8872389
TCAGATG29050.022.8005527
GTATTAG21400.018.5093671
TATACAG25700.017.93255
TAGGTAG10450.017.227325
GTATAAT31500.016.6899471
ATAGGAC10800.016.0025233
CTGTGCG5554.3673936E-815.5665359
TATACCG13950.015.4860645
TATACGG15150.015.2100745
AGTCCCG4752.3077682E-615.1600435
TACGGTG15300.015.0578257
CTATACT22750.014.8770694
Produced by FastQC (version 0.11.2)