FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p112c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p112c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235958
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG14350.044.665674
GCAGCGT14450.038.8827061
CAGCGTC17600.035.191272
AGCGTCA21200.030.2340683
CGTCAGA23700.027.0444035
GTCAGAT27700.023.3990636
CAGATGT29550.022.657588
TCAGATG31450.020.8309387
AGATGTG32150.020.8252419
ACCGCGC3251.052962E-619.9364228
TATACAG30450.017.0286795
GTATAGA24650.016.9488741
GTATTAG25100.016.931991
ATACAGG21250.016.60636
GTATATA64750.016.4646191
CTACACA16700.015.9558884
TATACGG19000.015.5405355
CATATAC38150.015.1021193
CCCACAC16400.014.9306093
TCTATAC25200.014.8609053
Produced by FastQC (version 0.11.2)