FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p111d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p111d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6386017
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG31200.043.3926474
GCAGCGT27550.042.875241
CAGCGTC33200.039.261512
AGCGTCA39050.034.4852563
CGTCAGA47300.029.0793765
GTCAGAT57600.024.629566
AGATGTG62500.023.614649
CAGATGT60250.023.5405528
TCAGATG67700.021.0563937
GTATTAG47300.015.9886751
GTACTAG28650.015.5865991
TATACAG59650.015.5737125
GTATATA119150.015.3540861
CTATACT49600.014.8091934
TAGGTAG23800.014.2211195
TAGACAG29550.014.1345965
ACTATGC40300.014.113338
TATATAC130300.014.0931693
GATGTGT54050.013.9862437
GTATAAT79650.013.9257711
Produced by FastQC (version 0.11.2)