FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mas4x58p110b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mas4x58p110b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7572942
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG33200.045.7651824
GCAGCGT34800.037.2525331
CAGCGTC42750.035.205892
AGCGTCA47350.032.0887873
CGTCAGA55600.027.9749815
CAGATGT68850.023.3184688
AGATGTG70150.023.2968549
GTCAGAT68250.023.2119086
TCAGATG72000.022.4982687
TATACAG81350.020.359225
GTATATA162800.018.093871
GTATTAG54750.017.8902871
ATACAGG59750.017.7161886
GAGCGCA18100.017.5014179
TATACCG37450.016.5362665
CTAATAC59000.015.5003863
TAGGTAG28700.015.3051925
TACAGGC40900.015.13827
TATAATA196350.014.9635432
TAGAGCG21350.014.8372657
Produced by FastQC (version 0.11.2)