FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mah58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mah58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408567
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG14950.037.5683674
CAGCGTC17250.031.7243982
GCAGCGT15650.030.3738921
AGCGTCA21200.026.4923973
CGTCAGA23750.024.5578485
CAGATGT28950.020.39258
AGATGTG29850.020.2600339
GTCAGAT29400.019.8383986
TCAGATG32200.018.1106647
GTATTAG23000.017.2228781
TATACAG33550.017.1698465
TACACCG10550.017.0630425
GTATAGA25750.017.0617471
TATACCG15750.016.9157035
TCTATAC26900.016.060593
CTATACT26700.015.3720754
TATATAC77200.015.1098543
GTATATG36350.014.464031
ACGGTGT16800.014.1419698
ATACCGT24450.014.1361556
Produced by FastQC (version 0.11.2)