FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mah4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mah4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3453253
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG16350.044.479194
GCAGCGT17150.038.2147641
CAGCGTC18850.038.1997762
AGCGTCA21950.033.1323853
CGTCAGA24700.030.317245
GTCAGAT30450.025.065246
AGATGTG32050.024.484729
CAGATGT32700.023.7778558
TCAGATG34400.022.8120737
GACCGCG3809.822543E-920.8403877
GTATTAG22950.020.0840471
TAGGGTG7350.017.6334975
ACCGCGC3703.6387282E-617.512078
TATACGG19500.016.985435
ACAGTGC12950.015.5662838
CTAGTAC13250.015.2162633
CTAATAC26500.015.2162633
ACTATGC19250.014.9598058
CATATAC37600.014.9373193
CTGAGCG6802.1955202E-914.8223089
Produced by FastQC (version 0.11.2)