FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mah33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mah33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125315
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG13000.034.3403554
GCAGCGT12600.030.8672391
CAGCGTC14750.029.7802942
AGCGTCA18900.023.2393763
CGTCAGA19850.022.8526445
CTGTGCG5200.022.1515249
TACACAG15800.018.2288535
CAGATGT25300.018.2115298
GTCAGAT25700.017.930956
GTATAGA23200.017.6954421
AGATGTG26900.017.6635769
TATACCG13550.017.5360225
GTATAGG14850.017.4602571
TACCGTG9900.017.4527157
GTCCTAA10850.017.2591021
TATACAG29350.015.9463645
TATACGG18100.015.9124795
TCAGATG28600.015.8584787
GTATAAT35600.015.7804421
GTATATA52450.014.9677181
Produced by FastQC (version 0.11.2)