FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mah148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mah148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3237213
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG16150.034.3329964
AGCGTCA17350.031.958383
CAGCGTC16850.030.7718072
GCAGCGT13850.030.6819531
CGTCAGA21100.027.6436945
GTCAGAT27200.022.2384196
AGATGTG28550.021.9377789
CAGATGT28300.021.8771888
TCAGATG32400.019.1087787
GTATATA59700.016.407611
TAGGTAG12900.015.6301045
TATACCG15350.015.4810025
ACTGTGC13100.015.3874348
GTATAAT41200.015.3838911
GTGTAGA18550.015.1426341
TATACAG31200.015.0021085
TAGACGG8701.8189894E-1214.8986445
CTGTGCG5909.253017E-814.642289
TATACGG17400.014.4847935
GTATAGA25000.014.4049181
Produced by FastQC (version 0.11.2)